Fig. 3: miR-6236 promotes insulin-mediated signaling and functions in adipocytes.

a AKT2 and pAKT2 Western blot and quantification in eWAT of DIO wild-type (+/+) or miR-6236 knockout (−/−) male mice (n = 4; p < 0.0001). b Insulin-stimulated glucose (Glu.) uptake in mature adipocytes isolated from eWAT of DIO +/+ (n = 5) or −/− (n = 4) male mice (p = 0.037). RLU Relative light units. c Glycerol release from eWAT adipocytes of DIO +/+ or −/− male mice in the presence of insulin (n = 4; p = 0.017). d Expression of key lipolysis genes in eWAT of DIO +/+ (n = 5) or −/− (n = 4) male mice (Lipa, p = 0.0016; Lipe, p = 0.1044; Lpl, p = 0.0011; Plin2, p = 0.032). e HSL and pHSL Western blot and quantification in eWAT of DIO +/+ (n = 3) or −/− (n = 4) male mice (p = 0.0003). f AKT2 and pAKT2 Western blot and quantification in +/+ primary adipocytes (Ad.) treated with EVs isolated from −/− or +/+ BMDMs (n = 4 technical replicates, p = 0.021). g Insulin-stimulated glucose uptake in +/+ primary adipocytes treated with EVs isolated from −/− or +/+ BMDMs (n = 3 technical replicates, p = 0.0033). h Glycerol release from +/+ primary adipocytes treated with EVs isolated from −/− or +/+ BMDMs (n = 6 technical replicates, p = 0.0018). i–j Fed and fasted (5 h) blood glucose (i) and serum insulin (j) levels (n = 10 mice/group, Fed Glucose, p = 0.016; Fed Insulin, p = 0.030) in DIO +/+ male mice injected i.p. with EVs from −/− or +/+ BMDMs. Data representative of ≥2 experiments. Data presented as mean ± SEM; ns not significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, two-tailed Student’s t test.