Fig. 1: Lyz-IFNγR2−/− mice are protected from obesity-mediated insulin resistance in the liver. | Nature Communications

Fig. 1: Lyz-IFNγR2−/− mice are protected from obesity-mediated insulin resistance in the liver.

From: IFNγ-IL12 axis regulates intercellular crosstalk in metabolic dysfunction-associated steatotic liver disease

Fig. 1: Lyz-IFNγR2−/− mice are protected from obesity-mediated insulin resistance in the liver.The alternative text for this image may have been generated using AI.

Metabolic and molecular experiments were performed in male Lyz-IFNγR2−/− and LysM-Cre+ (WT) mice at 6 ~ 7 months of age following 10 weeks of a high-fat diet (HFD; 7 WT and 8 Lyz-IFNγR2−/− mice) or a low-fat diet (LFD; 4 WT and 6 Lyz-IFNγR2−/− mice) serving as controls. A Body weight. B Whole-body fat mass, measured using 1H-magnetic resonance spectroscopy (MRS). C Basal plasma glucose levels after overnight fast. D Glucose infusion rate required to maintain euglycemia during a standardized 2-hour hyperinsulinemic-euglycemic clamp in awake mice. E Basal hepatic glucose production (HGP). F Clamp HGP during the insulin-stimulated state. G Hepatic insulin action expressed as insulin-mediated percent suppression of basal HGP. H Whole-body glucose turnover. I RT-qPCR analysis of liver glucose 6-phosphatase (G6Pase) and phosphoenolpyruvate carboxykinase (PEPCK) genes in HFD-fed mice (n = 4 HFD-WT and 5 HFD-Lyz-IFNγR2−/− mice). mRNA levels were normalized to HPRT as a housekeeping gene and are shown relative to HFD-WT mice. J Liver triglyceride levels (n = 4 LFD-WT, 5 LFD-Lyz-IFNγR2−/−, 8 HFD-WT, and 8 HFD-Lyz-IFNγR2−/− mice). K Liver protein expression of insulin receptor (InsR), insulin receptor substrate (IRS)-1, Akt, Ser473-phosphorylation of Akt, and forkhead box protein O1 (FoxO1) using the Jess Multiplexed Western Blot System in HFD-fed mice (n = 3 ~ 4 HFD-WT and 3 ~ 4 HFD-Lyz-IFNγR2−/− mice). Protein levels were normalized to β-actin as a loading control. Data are presented as mean ± SEM values. The statistical significance of the difference in mean values between Lyz-IFNγR2−/− mice and WT mice fed with a HFD or LFD was determined using a one-way analysis of variance (ANOVA) with Tukey’s multiple comparison test for post-hoc analysis (Fig. A-H and J). The statistical significance of the difference in mean values between HFD-fed Lyz-IFNγR2−/− mice versus HFD-fed WT mice was determined using a two-tailed Student’s t-test (Fig. I and K).

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