Fig. 3: Flowering characterizations of the BrJMJ18 transgenic Par plants under greenhouse and field conditions, respectively. | Nature Communications

Fig. 3: Flowering characterizations of the BrJMJ18 transgenic Par plants under greenhouse and field conditions, respectively.

From: Temperature-dependent jumonji demethylase modulates flowering time by targeting H3K36me2/3 in Brassica rapa

Fig. 3: Flowering characterizations of the BrJMJ18 transgenic Par plants under greenhouse and field conditions, respectively.The alternative text for this image may have been generated using AI.

a–h Phenotypes of the BrJMJ18 transgenic Par plants grown in the greenhouse under different temperature conditions. a BrJMJ18 transgenic Par plants grown under normal conditions (NC) for 9 weeks. Scale bar, 5 cm. b Phenotypes of the BrJMJ18 transgenic Par plants grown under NC conditions for 4 weeks, following another 5 weeks under high temperature (HT) conditions. Scale bar, 5 cm. Red arrows, withered leaves. c Flowering time of plants is shown in (a) and (b). DAS, days after sowing. Data are means ± SD, n = 15. Asterisks indicate significant differences between NC and HT, two-tailed Student’s t-test (***p < 0.001) Par, p = 2.53 × 10−5; BrJMJ18Par-OX, p = 9.28 × 10−9. d Confirmation of BrJMJ18 protein expression in the transgenic plants by immunoblotting analysis. Ponceau S staining was used to assess equal loading. e, f Flowering characterizations of BrJMJ18 knockout Par line, BrJMJ18Par-CR, in greenhouse under different temperatures. g Flowering time of plants shown in (e) and (f). Red arrows, withered leaves. Data are means ± SD, n = 15. Asterisks indicate significant differences between NC and HT, two-tailed Student’s t-test (***p < 0.001) Par, p = 2.32 × 10−10; BrJMJ18Par-CR, p = 1.47 × 10−16. The experiments in (a–f) were repeated three times with similar results. h Confirmation of BrJMJ18Par-CR by Sanger sequencing. A single base pair deletion (G → /) at the + 661 position after the ATG leads to a frame-shift mutation of the BrJMJ18 protein. i, j Par, BrJMJ18PC- and BrJMJ18Par-OX plants were planted in a tunnel greenhouse under natural field conditions in the summer (15th, Jul. … 29th, Sept.) of 2021 to simulate extremely high-temperature conditions for flowering time characterization. Data are means ± SD, n = 20. Asterisks indicate significant differences between Par and BrJMJ18Par-OX, two-tailed Student’s t-test (***p < 0.001), p = 8.19 × 10−5. For box plots (c, g, and j), the box encompasses two middle quartiles, with a central line showing the median. Whiskers extend to the furthest data point within 1.5 times the interquartile range. Source data are provided as a Source Data file.

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