Fig. 8: Amino acid mutations of T345A, Y633C and L654F contribute to functional divergence of BrJMJ18PC and BrJMJ18Par. | Nature Communications

Fig. 8: Amino acid mutations of T345A, Y633C and L654F contribute to functional divergence of BrJMJ18PC and BrJMJ18Par.

From: Temperature-dependent jumonji demethylase modulates flowering time by targeting H3K36me2/3 in Brassica rapa

Fig. 8: Amino acid mutations of T345A, Y633C and L654F contribute to functional divergence of BrJMJ18PC and BrJMJ18Par.The alternative text for this image may have been generated using AI.

(BrJMJ18Par(A345T) is identical to the sequence of BrJMJ18Par except with a substitution at position 345 at which A was replaced with T, which is specific to BrJMJ18PC; the same goes for BrJMJ18Par(C633Y), BrJMJ18Par(F654L), and BrJMJ18Par(C633Y)/(F654L)). BrJMJ18PC-GFP, BrJMJ18Par-GFP, and its variant proteins were transiently overexpressed in tobacco leaves. H3K36me3 and H3K36me2 were detected using anti-H3K36me3 and anti-H3K36me2 antibodies in 35S::BrJMJ18-GFP expressing leaves under NC for 3 days and NC for 2 days following 1-day of HT, respectively. BrJMJ18-GFP proteins were detected with anti-GFP antibodies to confirm equal expression of exogenous genes. Ponceau S staining was used to assess equal loading of total protein extracts. All the western blot experiments were repeated at least three times with similar results. Source data are provided as a Source Data file.

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