Fig. 2: Acute stimulus-evoked effects of psilocin on PVN reactivity and behavior in males. | Nature Communications

Fig. 2: Acute stimulus-evoked effects of psilocin on PVN reactivity and behavior in males.

From: Increased reactivity of the paraventricular nucleus of the hypothalamus and decreased threat responding in male rats following psilocin administration

Fig. 2

Fiber photometry: Psilocin n = 9, Vehicle n = 9; Behavior: Psilocin n = 11, Vehicle n = 12; A Baseline PVN reactivity (left): ΔF/F trace plots of changes following 500 ms air-puff. A 2-way ANOVA was performed for statistical analysis. Data points represent 500 ms binned group averages with mean +/- S.E.M (shaded area).; Heatmaps (top right) comparing individual responses to air-puff (dotted line) in vehicle and psilocin groups.); Average AUC and PP + /- S.E.M. (bottom right) compared by 2-tailed unpaired t-tests between groups. Each data point represents an individual subject. B Average maximum speed (left) and distance traveled (right) after air-puff stimulus. A 2-way ANOVA was performed for statistical analysis. Data points are group averages +/- S.E.M. (shaded area) (C) Day of injection PVN reactivity (left): ΔF/F trace plots of changes following 500 ms air-puff. A 2-way ANOVA found a significant treatment x time interaction (p = 0.0092) and a main effect of treatment (p = 0.0340). Šidák multiple comparisons found significant differences at time bin 13 (p = 0.0149). Data points represent group averages within 500 ms binned window +/- S.E.M. (shaded area); Heatmaps (top right) comparing individual responses to air-puff (dotted line) in vehicle and psilocin groups. Average AUC and PP + /- S.E.M. (bottom right) compared by 2-tailed unpaired t-test with a significant difference in AUC (p = 0.0129) and PP (p = 0.0330) between groups. Each data point represents an individual subject. D Average maximum speed (left) and distance traveled (right)after air-puff stimulus. A 2-way ANOVA found a main effect of treatment (p = 0.0208). Data points are group averages +/- S.E.M (shaded area). In each trace bin plot, a significant increase in ΔF/F was determined whenever the lower bound of the 99% CI was >0 with statistical significance shown as colored lines above each ΔF/F curve with colors corresponding to the respective binned traces. *p < 0.05, **p < 0.01, *** p < 0.001, **** p < 0.0001. AUC = area under curve, PP = peak point, ΔF/F = change in fluorescence as a function of baseline fluorescence, CI = confidence interval.

Back to article page