Fig. 8: Psilocin produces increases in PVN reactivity in active responding males. | Nature Communications

Fig. 8: Psilocin produces increases in PVN reactivity in active responding males.

From: Increased reactivity of the paraventricular nucleus of the hypothalamus and decreased threat responding in male rats following psilocin administration

Fig. 8

Fiber photometry: Psilocin active n = 4, Vehicle active n = 5, Psilocin passive n = 5, Vehicle passive n = 4; Behavior: Psilocin active n = 4, Vehicle active n = 6, Psilocin passive n = 7, Vehicle passive n = 6; (A) Baseline PVN reactivity (left): ΔF/F trace plots of changes following exposure to a 500 ms air-puff. 2-way ANOVA was performed for statistical analysis. Data points represent group averages within 500 ms binned window +/- S.E.M. (shaded area); Heatmaps (top right) comparing individual responses to air-puff stimulus (dotted line) in vehicle and psilocin groups. Average AUC and PP + /- S.E.M. (bottom right) compared by unpaired 2-tailed t-test between groups. Each data point represents an individual subject. B Average maximum speed (left) and distance traveled (right) following air-puff stimulus. 2-way ANOVA was performed for statistical analysis. Data points are group averages +/- S.E.M. (shaded area) (C) PVN reactivity (left): ΔF/F trace plots of changes following exposure to a 500 ms air-puff. 2-way ANOVA significant treatment x time interaction (p < 0.0001) and main effect of treatment (p = 0.0091). Data points represent group averages within 500 ms binned window +/- S.E.M. (shaded area); Heatmaps (top right) comparing individual responses to air-puff stimulus (dotted line) in vehicle and psilocin groups. Average AUC and PP + /- S.E.M (bottom right) compared by unpaired 2-tailed t-test between groups, AUC (p = 0.0202). Each data point represents an individual subject. Inset: Histogram reflecting change in the proportion of active responding animals compared to baseline. 2-sided Fisher’s exact test revealed a significant difference between groups (p = 0.0004). D Average maximum speed (left) and distance traveled (right) following air-puff stimulus. 2-way ANOVA was performed for statistical analysis. Data points are group averages + /- S.E.M. (shaded area). In each trace bin plot, a significant increase in ΔF/F was determined whenever the lower bound of the 99% CI was >0 with statistical significance shown above each ΔF/F curve with colors corresponding to the respective binned traces *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. AUC = area under curve, PP = peak point, ΔF/F = change in fluorescence as a function of baseline fluorescence, CI = confidence interval.

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