Fig. 3: Single-cell analysis of lung and peripheral blood samples from patients treated with or without dexamethasone.
From: Distinct pulmonary and systemic effects of dexamethasone in severe COVID-19
Plot per patient showing the collection of whole blood (WB) (a N = 7 Dex, 3 NoDex) or tracheal aspirate (TA) samples (b N = 10 Dex, 7 NoDex) overlaid on hospitalization (gray bars) and dexamethasone treatment (pink bars). X-axis shows days of hospitalization (day 0 = admission to UCSF hospital). Dots show the day when sample was collected, colored by Study Day (methods). UMAP plots of single-cell RNA-seq data from blood (c) or TA (d) samples, clustered and annotated by major immune cell types. UMAP plots of single-cell RNA-seq data from blood (e) or TA (f) samples, colored by Dex (blue) or NoDex (pink) samples. log2 fold difference of gene expression of Dex and NoDex in TA (y-axis) v. blood (x-axis) plotted for Neutrophils (g) and Tregs (h). Significant genes in TA only (blue), blood only (brown), both compartments (red) are shown (BH-adj. p < 0.1 & |log2 fold-difference | > 0.5). Spearman’s correlation R value shown between the two compartments.
