Fig. 4: The sequence of virBR gene and upstream region, and key amino acids of VirBR.
a Sequence schematic diagram of virBR with upstream region. Predict promoter and TSS are shown in red font. Sequence of virBR is shown in green font. b The predicted secondary structure of VirBR using psipred. The α-helices and β-strands are shown as pink and yellow cylinders, respectively. c The predicted protein structure of VirBR using AlphaFold. The key amino acid was shown in Arg 20, Arg 42 and Arg 67. d Amino acid substitution analysis on conjugation frequency comparing WT VirBR (black bar) to individual substituted arginine residues (Arg 20, 42 and 67) with either alanine (A) or glutamic acid (E). Data are means ± SEM; n = 3 biologically independent replicates. Groups were compared using two-tailed t-test. e Fluorescence intensity of PactX-EGFP with each of the constructs described in d (red bars) or without VirBR (black bar). Data are means ± SEM; n = 4 biologically independent replicates. Groups were compared using two-tailed t-test. Source data are provided as a Source Data file.
