Fig. 1: HFD feeding decreases RORγt⁺ pTregs and Th17 cells in the small intestine and colon.

A–D Body weight (n = 13–14 mice per group) (A), body weight gain (n = 13–14 mice per group) (B), epididymal fat mass (n = 13–14 mice per group) (C) and body composition (fat and lean mass) (n = 5–6 mice per group, statistical significance assessed with 2-way ANOVA and Sidak’s multiple comparison test, stars display adjusted P value) (D) in wild-type mice fed a chow diet (CD) or a high-fat diet (HFD) for 4 weeks. E, F Oral glucose tolerance test and associated area under the curve (AUC) quantification (E), HOMA-IR index measurement (F), fasted plasma glucose (G), and insulin (H) levels in wild-type mice fed a chow diet (CD) or a high-fat diet (HFD) for 4 weeks (n = 13–14 mice per group). I–K Colon weight (n = 13–14 mice per group) (I), colon length (n = 9–10 mice per group) (J) and cecum weight (n = 13–14 mice per group) (K) in wild-type mice fed a chow diet (CD) or a high-fat diet (HFD) for 4 weeks. L Flow cytometry plots depicting RORγt⁺ CD4 T cells, including RORγt⁺ pTregs and Th17 cells, in the small intestine of chow diet-fed wild-type animals. M, N Flow cytometry analysis of RORγt⁺ pTregs (M) and Th17 cells (N) in the mesenteric lymph nodes (mesLNs) (n = 3 mice), the small intestine (n = 6 mice) and the colon (n = 5 mice) of chow diet-fed wild-type animals. Statistical significance tested with 1-way ANOVA and Newman-Keuls multiple comparison test. O–Q Flow cytometry analysis of total Tregs (O), RORγt⁺ pTregs (P) and Th17 cells (Q) in the small intestine and colon of wild-type mice fed a chow diet (CD) or a high-fat diet (HFD) for 4 weeks (n = 8–9 mice per group). All data in this figure are presented as mean values ± SEM. All panels correspond to two independent experimental groups. Statistical significance has been assessed with a two-sided T test unless otherwise stated on the corresponding panel legend.