Table 1 Parameter setting in Sutter P−1000 micropipette puller (model P−1000; Sutter Instruments Co., US)

From: Microscale geometrical modulation of PIEZO1 mediated mechanosensing through cytoskeletal redistribution

Tip angle θ

Stage

Heat

Pull

Velocity

Time

Pressure

Repetition

0°

1

Ramp+10

150

75

250

500

once

5°

1

Ramp+14

0

90

255

 

once

2

Ramp+14

0

32

255

670

twice

3

Ramp+4

0

37

255

 

once

10°

1

Ramp+14

0

90

255

 

once

2

Ramp+14

0

32

255

670

twice

3

Ramp+4

0

29

255

 

once

  1. All parameters are defined based on the application, slight modification is required for every new setup. Ramp value was measured based on the heating filament in the puller.
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