Fig. 1: Structural characterization of a cCMP-synthesizing PycC cyclase, EaPycC.
From: Structural and functional characterization of cyclic pyrimidine-regulated anti-phage system
a, b Cartoon representation of (a) protomer and (b) dimer structure of EaPycC. The N- and C-terminus are labeled. The symmetric active sites of EaPycC dimer are indicated by black dashed circles and the conserved catalytic residues D102 and D146 are shown in ball-and-stick model. c The front (left) and back (right) view of the superimposed EaPycC and BcPycC promoters. The missing residues (a.a. 22–66) of EaPycC protomer are indicated by black dashed lines. The inlet shows the enlarged view of the superimposed Y-shaped structure of EaPycC with β-hairpin of BcPycC at the equivalent position. d, e Structural alignment of EaPycC dimer with (d) BcPycC dimer, and (e) PaPycC dimer. EaPycC, BcPycC, and PaPycC proteins are colored in salmon, cyan, and pale-green, with their distinct N- and C-terminal structural elements colored in red, blue, and forest-green respectively.
