Fig. 6: DCAF15 loss results in enlargement of chromatin loops.
From: DCAF15 control of cohesin dynamics sustains acute myeloid leukemia
a Contact probability as a function of genomic distance in Cas9+ HEL cells infected with lentiviruses encoding ROSA26-targeting (negative control) or DCAF15-targeting sgRNAs. Hi-C data derived from n = 2 independent biological replicates in each condition. b Representative region in chromosome 6 (chr6:05,000,000–11,500,000) showing contacts, distribution of ac-SMC3, and sites with differential acetylation of SMC3. Black boundaries delineate TADs called in each condition. Arrows indicate domain boundaries lost in DCAF15−/− cells. c 3D pileup plots of TADs in each condition and log2(Fold Change) upon loss of DCAF15. d Box plot showing length distributions of loops called in DCAF15+/+ versus DCAF15−/− cells. n = 10,662 loops (sgROSA26) and 11,871 loops (sgDCAF15#2). Center line shows median, box limits show 75th and 25th percentiles, whiskers show 1.5X interquartile range (IQR); outliers not shown. sgROSA26 median loop length: 225 kb, sgDCAF15#2 median loop length: 310 kb. Welch’s two-sided t-test, ****p-value < 0.0001 (p = 2.2 × 10−16). e 3D pileup plots of loops categorized by indicated size ranges in each condition and log2(Fold Change) upon DCAF15 loss. f Representative region in chromosome 9 (chr9:14,000,000–18,000,000) showing contacts, distribution of ac-SMC3, and sites with differential acetylation of SMC3. Loops present in control only or in both conditions are indicated in blue. Loops gained in DCAF15-knockout condition only are indicated in red. Arrows indicate example regions of increased contact in DCAF15−/− cells.
