Fig. 1: Functional characterization and structure determination of hSVCT1.
From: Dimeric transport mechanism of human vitamin C transporter SVCT1
a Time-dependent ascorbic acid transport into HEK293T cells by hSVCT1 (n = 3 biological replicates). b Km curve for hSVCT1-derived ascorbic acid uptake into HEK293T cells (n = 5 for hSVCT1, n = 3 for mock). Source data are provided as a Source Data file. c Cryo-EM map of the hSVCT1 homodimer in the inward-open state, with densities corresponding to each protomer and lipids colored blue, gray, and pink, respectively. d Cryo-EM map of the hSVCT1 homodimer in the substrate-free occluded state, with densities corresponding to each protomer and lipids colored orange, gray, and pink, respectively.
