Fig. 2: Impaired trophoblast stemness and differentiation upon Kat8 deletion.

a Schematic showing generation of Kat8^d/d mTSCs. The graphic elements were created by figdraw. b Immunoblot analysis of KAT8 and H4K16ac in mTSCs treated with or without Dox for 48 h (Kat8^d/d and Kat8^f/f). c Representative immunofluorescent staining and brightfield pictures of H4K16ac and Ki67 in Kat8^d/d and Kat8^f/f mTSCs. d The quantitative results of C. Data are representative of three independent experiments (n = 3) and the values are normalized to Kat8^f/f control group. Two-tailed unpaired Student’s t-test. Error bars, mean ± SEM. H4K16ac, P < 0.0001. Ki67, P < 0.0001. Source data are provided as a Source Data file. e Volcano plot of differentially expressed genes (P < 0.05, Log₂FC ≥ 1) in Kat8^f/f and Kat8^d/d mTSCs as determined by exact negative binomial test in edgeR package of R. f Gene Ontology analysis for Top 150 differentially downregulated genes by DAVID by Kappa Statistics (p < 0.05). g GSEA plot showing the enrichment of placenta development related genes in Kat8^d/d compared with Kat8^f/f mTSCs. NES normalized enrichment score. P = 0.02. h Heatmap of mTSCs stemness associated genes between Kat8^f/f and Kat8^d/d mTSCs. i Quantitative real-time PCR analysis of Cdx2, Elf5, Ly6a, and Eomes mRNA levels in Kat8^f/f and Kat8^d/d mTSCs. Data are representative of three independent experiments (n = 3) and the values are normalized to ACTB. Two-tailed unpaired Student’s t-test. Error bars, mean ± SEM. Cdx2, P < 0.0001. Elf5, P < 0.0001. Ly6a, P < 0.0001. Eomes, P < 0.0001. Source data are provided as a Source Data file.