Fig. 8: TOGARAM2 is pro-oncogenic in HPV18+ cervical cancer cells.
From: The Hippo pathway transcription factors YAP and TAZ play HPV-type dependent roles in cervical cancer
A RT-qPCR analysis of TOGARAM2 expression in TOGARAM2 KD HeLa cells (n = 3). U6 was used as a loading control. B Growth curve analysis of TOGARAM2 KD HeLa cells (n = 3). C Colony formation assays in TOGARAM2 KD HeLa cells (n = 4). D Soft agar assays in TOGARAM2 KD HeLa cells (n = 4). E Immunofluorescence microscopy analysis of Rhodamine-Phalloidin stained (red) TOGARAM2 KD HeLa cells. DAPI stained nuclei (blue). Scale bar 10 µm. F Measurement of filopodia length in TOGARAM2 KD HeLa cells (n = 10). G Average number of filopodia per cell in TOGARAM2 KD HeLa cells (n = 10). Error bars represent the mean +/- standard deviation of a minimum of three biological repeats. *P < 0.05, **P < 0.01, ***P < 0.005 (two-tailed, unpaired Student’s t-test). Source data are provided as a Source Data file.
