Fig. 4: Profiling of porcine brain GSLs at the C = C location level. | Nature Communications

Fig. 4: Profiling of porcine brain GSLs at the C = C location level.

From: Illuminating the dark space of neutral glycosphingolipidome by selective enrichment and profiling at multi-structural levels

Fig. 4

a Generic structures of C = C diagnostic ions generated from charge-tagging PB-MS2 CID. b EIC of HexCer 18:1;O2/23:1 and HexCer 18:2;O2/23:0 derivatized by 2-acpy ([PBM + H]+, m/z 917.5). MS2 CID spectra for peaks eluted at c 18 min and d 21 min in panel b. e MS2 CID spectrum of HexCer 18:1;O2/23:1;2OH derivatized by 2-acpy ([PBM + H]+, m/z 933.5). f %Relative composition of C = C location isomers detected in HexCer 18:1;O2/23:1 and HexCer 18:1;O2/23:1;2OH. g MS2 CID spectrum of HexCer 18:1;O2/26:4, 2OH derivatized by 2-acpy ([PBM + H]+, m/z 969.5). Source data are provided in the Source Data file.

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