Fig. 3: OriC and replisome displacements at different cell areas.
From: The Escherichia coli chromosome moves to the replisome
a, b Frame-to-frame displacement of tracked fluorescent foci between two frames acquired 1 s apart. Foci tracking was performed using the u-track33 algorithm. Displacements at different cell sizes estimated for (a) oriC-proximal chromosome loci and (b) replisomes. Heat map color shows the probability density for different displacements in each area bin. c Displacement distributions from (a, b) at specific cell sizes. d 2D distances between the oriC-proximal locus and replisomes for different cell areas. Heat map grayscale shows the probability density for different distances in each area bin. a, b, d: Red dashed lines indicate average replication initiation size. The experiment was performed twice. Statistics can be found in Supplementary Dataset 1.
