Fig. 4: Optogenetic activation of PrL GABAergic projections in the Cg1/2 increases the response to mechanical stimulation. | Nature Communications

Fig. 4: Optogenetic activation of PrL GABAergic projections in the Cg1/2 increases the response to mechanical stimulation.

From: Long-range inhibition from prelimbic to cingulate areas of the medial prefrontal cortex enhances network activity and response execution

Fig. 4

a Mice were injected with virus (AAV-DIO-ChR2-mCherry, N = 9 mice, AAV-DIO-YFP, N = 9 mice) into the PrL and an optic fiber was implanted into the Cg1/2. They were subsequently tested in a mechanical stimulation assay using von Frey filaments (session 1, pre-optical stimulation; session 2, peri-optical stimulation; session 3, post-optical stimulation) each with 6 different vF filament forces. b There is no difference in basal mechanical sensitivity between ChR2 and YFP groups prior to optical stimulation (all p > 0.05). c During optical stimulation of PrL GABAergic axon terminals in the Cg1/2 (10-ms pulses at 20 Hz, 473-nm wavelength), there is a group v force interaction (F(5,80) = 3.57, p = 0.0058) and an increased sensitivity to the 0.16-g (p < 0.0001) and 0.4-g filaments (p = 0.0003). d Following optical stimulation, there is again no difference between the ChR2 and YFP groups in response to vF stimulation (all p > 0.05). All data were analyzed by RM ANOVA followed by two-sided Bonferroni corrected multiple comparisons. Lines on graphs show group means ± S.E.M., ***p ≤ 0.001.

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