Fig. 7: TWEAK/Fn14-driven NAMPT regulates NAD + /NADH and ATP production to stimulate filopodia and promote invasion in TNBC cells. | Nature Communications

Fig. 7: TWEAK/Fn14-driven NAMPT regulates NAD + /NADH and ATP production to stimulate filopodia and promote invasion in TNBC cells.

From: TWEAK/Fn14 signalling driven super-enhancer reprogramming promotes pro-metastatic metabolic rewiring in triple-negative breast cancer

Fig. 7

a Plot depicting the relative NAD + /NADH levels in control and NAMPT enhancer #3 deleted MDA-MB-231 cells, with and without TWEAK treatment. b Plot depicting the relative intracellular ATP levels in control and NAMPT enhancer #3 deleted MDA-MB-231 cells, following TWEAK and NAD+ treatment. c Representative images of control and NAMPT enhancer #3 deleted MDA-MB-231 cells stained for actin and DAPI, following TWEAK, NAD+ and LatA treatment, using a Zeiss Live Cell Observer microscope (63x magnification). White arrows point to filopodia protrusions. Scale bars: 50 µm. (n = 3 biological replicates) (d) Plot depicting the average filopodia density (mean ± s.d) from n = 3 biological replicates. e Representative images of transwell invasion assay performed in Control and NAMPT enhancer #3 deleted MDA-MB-231 cells following TWEAK, NAD+ and LatA treatment (n = 3 biological replicates). Scale bars: 400 µm. f Plot depicts the average number of invaded cells/frame (mean ± s.d) from n = 3 biological replicates, across four fields per replicate. Experiments involving cell lines were performed 3 times independently, each time on different days. Two-sided one-way ANOVA was used for statistical analysis all assays. *P < 0.05; **P < 0.01; ***P < 0.001. Source data are provided as a Source Data file.

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