Fig. 2: Wild-type animals enter into a diapause state at 4 °C.
From: Regulatory mechanism of cold-inducible diapause in Caenorhabditis elegans
a Eggs were unhatched at 4 °C and not rescued by warming to 20 °C after cold exposure. b–d Cold treatment at 4 °C induced diapause in wild-type animals and hsf-1(sy441) mutants. hsf-1(sy441) mutants exhibited delayed development and morphological abnormalities after recovery from diapause induced at 4 °C. d Recovery experiments were repeated five times with more than 100 animals. Each dot indicates the day reproductive adults appeared on plates. The bars indicate the mean ± standard deviation (SD). n ≥ 3 biological replicates with more than 50 embryos and animals (a–c). Scale bars, 200 μm (a, c) and 1 mm (b). Arrows indicate L1 larvae and arrested worms (b), and protruding vulva (c). Source data are provided as a Source Data file.
