Fig. 3: IFN-Is promote OXPHOS and ATP production in tumor cells.

a, b WT MC38 cells were treated with IFN-α for 48 hours. Mitochondrial mass (a) and membrane potential (b) were measured by FACS (n = 3 biologically independent samples per group). c The ratio of MDR to MG was shown. d OCR was measured by Seahorse assay (n = 3 biologically independent samples per group). e Intracellular ATP concentration was measured (n = 3 biologically independent samples per group). f The expression of OXPHOS related genes were measured by real-time PCR (n = 3 biologically independent samples per group). g, h Ndufs6 KO MC38 cells were treated with IFN-α for 48 hours. Concentration of intracellular ATP (n = 3 biologically independent samples per group) (g) and extracellular ATP (n = 4 biologically independent samples per group) (h) were measured. i C57BL/6 mice (n = 5 mice per group) bearing WT or Ndufs6 KO MC38 tumors were treated i.t. with CV-1 or hIgG every three days. Tumor volume was measured at indicated time. Data are representative of four independent experiments in (a)–(c), (e), (g), and (h), three independent experiments in (d), two independent experiments in (f), i Two-tailed unpaired Student’s t test was used in (a)–(f). i Two-way ANOVA and multiple comparisons test was used in (g) and (h). Data are presented as mean values ± SEM. Source data are provided as a Source Data file.