Fig. 2: Functional characterisation of VtlG (P450). | Nature Communications

Fig. 2: Functional characterisation of VtlG (P450).

From: Iron-sulphur protein catalysed [4+2] cycloadditions in natural product biosynthesis

Fig. 2

a AlphaFold267 predicted the structure of VtlG. The haem cofactor shown in cyan was modelled from PDB 6J85 as a template. b Compound 4 accumulated in the vtlG disruptant, and compound 5 nonenzymatically converted from 4 during isolation. Time-dependent conversion of 1 μM 4 into 1 in vitro in the presence of 0.5 μM VtlG, 0.1 mg/mL spinach Fd (c) or 0.1 mg/mL VtlF (d), 1 unit/mL spinach FNR, and 100 μM NADPH. The reactions were performed in 50 mM Tris-HCl (pH 7.5) at 30 °C, and the reaction products were monitored by UPLC–MS.

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