Fig. 1: Cell-type specific transcriptional profiling of young Arabidopsis ovules.
From: Germline β−1,3-glucan deposits are required for female gametogenesis in Arabidopsis thaliana
a, b pKNU:YFPNLS ovule, the yellow fluorescent protein is detected solely in the megaspore mother cell (mmc) nucleus. c, d pWUS:GFP-WUS ovule, the WUS-GFP fusion protein is detected in the nucellus epidermis (ne). e, f pSTK:STK-GFP reporter protein is observed in the somatic cells of the ovule, in the nucellus epidermis (ne), chalaza (ch) and funiculus (f). Protoplasts produced from pKNU:YFPNLS (g, h), pWUS:WUS-GFP (i, j), and pSTK:STK-GFP (k, l) ovules showing fluorescent protein expression restricted to the nucleus. a, c, e Images result from merging bright-field DIC with YFP/GFP fluorescence. b, d, f Fluorescence channel only. Scale bars: 10 μm. RNAseq quality assessment: m Hierarchical clustering dendrogram and n Principal Component Analysis (PCA) scatter plot using read count matrixes calculated by DESeq2 method. o Venn diagram showing the number of genes expressed in each cell type (in parenthesis) and the overlap between transcriptomes. p–r Volcano plots depicting the differentially expressed genes for each comparison (blue: downregulated genes = FDR < 0.05, log2 (fold change) < −2; red: upregulated genes = FDR < 0.05, log2 (fold change) > 2). y axis - adjusted p-value is the FDR value calculated as described in the methods section. M35, M46, M79 = pKNU:YFPNLS samples (MMC) biological replicates; N05, N26, N39 = pWUS:GFP-WUS (NUC) biological replicates; S35, S47, S68 = pSTK:STK-GFP (STK) biological replicates.
