Fig. 3: Localisation of cell autonomous and mobile fluorophores in wild-type and pKNU:GLUC ovules.
From: Germline β−1,3-glucan deposits are required for female gametogenesis in Arabidopsis thaliana
a, b In wild-type ovules, cell-autonomous pAGO5:YFPER accumulates in the nucellar epidermis and inner integuments. c, d pAGO5:mStrfree shows a broader pattern in the chalaza and nucellus but is excluded from the MMC and is absent in the inner integuments. e, f A similar pattern is observed in pKNU:GLUC ovules. g, h In wild-type ovules, cell-autonomous pKNU:YFPNLS accumulates in the nucleus of the MMC. i, j. pKNU:mStrfree spreads throughout the MMC but does not spread into the nucellar epidermis of wild-type ovules. k, l A similar pattern is observed in pKNU:GLUC ovules in the majority of cases. m-r In approximately 18% of pKNU:GLUC ovules, pKNU:mStrfree signal is apparent outside of the MMC in adjoining nucellus and chalaza cells (arrows in n, p and r). Experiments were repeated on 5 independent occasions with similar results. Representative micrographs are shown. Scale bars = 20 µm. ch chalaza, f funiculus, ii inner integument, mmc megaspore mother cell, ne nucellar epidermis, oi outer integument.
