Fig. 4: Localisation of HPTS tracer in wild-type, pKNU:GLUC and pWUS:GLUC ovules.
From: Germline β−1,3-glucan deposits are required for female gametogenesis in Arabidopsis thaliana
a–f In wild-type, HPTS accumulates in the funiculus and chalaza of pre-meiotic and post-meiotic ovules. g–l A similar localisation pattern is observed in pKNU:GLUC ovules. m–r In pWUS:GLUC ovules, HTPS accumulates strongly in the funiculus and chalaza, progressing further towards the nucellus. Unlike the other tested genotypes, signal is occasionally detected in the vicinity of the mmc/megaspores. The red dashed lines indicate the interface between different ovule domains. Experiments were repeated on 4 independent occasions with similar results. Representative micrographs are shown. Scale bars = 20 µm. ch chalaza, dm degenerating megaspores, fu funiculus, ii inner integument, mmc megaspore mother cell, mt megaspore tetrad, ne nucellar epidermis, nu nucellus. oi outer integument.
