Fig. 6: PARP1 and PARG regulate biomolecular condensates containing Polθ and DNA.
From: PARG is essential for Polθ-mediated DNA end-joining by removing repressive poly-ADP-ribose marks
a–c Confocal images showing that PARP1 + NAD+ promote biomolecular condensates containing Cy3-DNA and Red-Polθ-pol. Confocal microscopy images are shown of Red-Polθ-pol and Cy3-DNA under the indicated conditions following Cy3 and Cy5 fluorescence as indicated. 10 μm bars are indicated. d, e Plots showing circularity (d) and size (e) of particles formed under the indicated conditions. Red circles represent particles with circularity scores >0.9 (d). f Confocal images showing that Red-Polθ-pol colocalizes with Cy3-DNA in droplets formed by PARP1 + NAD+. Confocal microscopy images are shown of Red-Polθ-pol and Cy3-DNA under the indicated conditions1,2,3,4 following Cy3 and Cy5 fluorescence as indicated. 10 μm bars are indicated. g Quantitation of the number of co-localization events between Red-Polθ-pol and Cy3-DNA under the indicated conditions. Co-localization events from 4 separate confocal microscopy images are quantitated. h Model of Polθ TMEJ regulation by PARP1 and PARG. Experiments in 6a–c, f were performed at least twice. Source data are provided as a Source Data file.
