Fig. 7: Temporal TMEJ activity in irradiated cells.
From: PARG is essential for Polθ-mediated DNA end-joining by removing repressive poly-ADP-ribose marks
a Schematic outline of TMEJ in vitro assay. Insert: Polθ inhibitor ART558 abrogated TMEJ activity (cells were treated with 12.5 μM ART558 for 16 h before IR and during incubation procedure) in duplicates from three independent biological replicates. ****p < 0.0001 using one-way ANOVA (p value 1 vs 2 p < 0.00001, 2 vs 3 p < 0.00001). b Mean number ± SD of XL-10 Gold colonies obtained from the in vitro TMEJ assay using nuclear lysates from U2OS cells obtained at 0, 20, 60 and 120 min. after 10 Gy irradiation (IR) or without IR from two independent experiments; ***p < 0.001 and ****p < 0.0001 using one-way ANOVA (p value 1 vs 6 p = 0.00012, 1 vs 7 p < 0.00001). c Representative images of XL-10 Gold colonies harboring the repaired pBABE-hygro-MMEJ plasmid. Source data are provided as a Source Data file.
