Fig. 1: HAC-seq analysis of METTL2A/2B- and METTL6-dependent m3C methylomes. | Nature Communications

Fig. 1: HAC-seq analysis of METTL2A/2B- and METTL6-dependent m3C methylomes.

From: m3C32 tRNA modification controls serine codon-biased mRNA translation, cell cycle, and DNA-damage response

Fig. 1

a Heat map showing the cleavage ratios of m3C modification sites detected by HAC-seq from WT, METTL2A/2B KO (M2KO), METTL6KO (M6KO), and METTL2A/2B/6 KO (M2,6KO) HEK293T cells. Data is shown as the mean value from two biological replicates. b–d A representative read alignments of tRNAs from untreated control (ctrl) and HAC-treated (HAC) groups of WT, M2KO, M6KO, and M2,6KO cells are shown in the Integrative Genomics Viewer (IGV). Representative plots of METTL2A/2B-prefered tRNAs (tRNA-Arg and tRNA-Thr) are shown in (b). Representative IGV plots of METTL6-prefered tRNAs (tRNA-Ser) are shown in c where m3C32 of tRNA-Ser-GCT is completely depleted only when METTL2A/2B/6 is knocked out. Orange labels shown in b and c represent sequencing mutation that reflects adenosine to inosine (A–I) editing at position A34. Representative IGV plots of METTL2A/2B/6-independent m3C modification sites are shown in (d). Source data are provided as a source data file.

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