Fig. 2: PV-neuron distribution is altered along with development.
From: Cortical parvalbumin neurons are responsible for homeostatic sleep rebound through CaMKII activation
a Whole-brain analysis of PV+ cell distribution during post-weaning development. P21, P28, P35, P42, P84, and P270 mouse brains were collected (n = 6 for each age). Each set of whole-brain images was uploaded to the CUBIC-Cloud and registered to the CUBIC-Atlas brain. Image of CUBIC-Atlas brain was from the CUBIC-Cloud. b The mean of PV+ cell density in the whole brain (top), cerebral regions (middle), and brain-stem regions (bottom) across post-weaning development (n = 6 for each age). Each point represents the mean value of each age normalized by P21. c Representative whole-brain views of PV+ cell distribution from the dorsal and lateral sides at P28 and P84 ages. d A virtual cortical slice depicts a voxel-wise P-value heat map of PV+ cell density in comparison to P28 and P84 brains. P28 and P84 brains were tested using two-sided Welch’s t-test. Red and blue colors represent a significant increase and decrease in P84 brains compared to P28 brains, respectively. Gray was assigned to regions with no significance (P > 0.05). e A region-wise heat map of relative cell number depicting changes in PV+ cell number in the isocortex. Red and blue colors represent a significant increase and decrease in P84 brains compared to P28 brains, respectively. f Mean PV+ cell density across post-weaning development in selected cortical regions (n = 6 for each age). Each point represents the mean value of each age normalized by that of P21. Images of coronal brain slice were from the Allen Brain Atlas. Line plots show mean ± SEM. The Allen Brain Atlas ontology is used to define brain region acronyms. Source data are provided as a Source Data file.
