Fig. 6: Inhibition of CaMKII activity in E11 neurons inhibits rebound in slow-wave activity.

a Diagram of CN19o expression in E11 neurons for CaMKII inhibition. b Hourly normalized delta power (0.5–4 Hz) during NREM sleep in E11-CN19scr (n = 22) and E11-CN19o mice (n = 15) in SD experiments. Mice were allowed to behave freely for 1 day prior to SD, and the value is shown as Basal. SD was effectively performed during ZT0–6 with a high success rate (93.4 ± 5.3%). c Normalized delta power during NREM sleep on Basal and SD during ZT6–12 in E11-CN19scr (n = 22) and E11-CN19o mice (n = 15). Two-sided Welch’s t-test was performed between Basal and SD within the groups. d The percentage of delta power change upon SD during ZT6–12 in E11-CN19scr (n = 22) and E11-CN19o mice (n = 15). The groups were compared using two-sided Welch’s t-test. e Diagram of CN19o expression in cortical PV neurons with enhanced specificity using E11 enhancer and Pvalb−2A-Cre mice. f Hourly normalized delta power (0.5–4 Hz) during NREM sleep in Pvalb−2A-Cre:E11-DIO-CN19scr (n = 16) and Pvalb−2A-Cre:E11-DIO-CN19o mice (n = 13) in SD experiments. Mice were allowed to behave freely for 1 day prior to SD, and the value is shown as Basal. SD was effectively performed during ZT0–6 with a high success rate (95.0 ± 6.2%). g Normalized delta power during NREM sleep on Basal and SD during ZT6–12 in Pvalb−2A-Cre:E11-DIO-CN19scr (n = 16) and Pvalb−2A-Cre:E11-DIO-CN19o mice (n = 13). Two-sided Welch’s t-test was performed between Basal and SD within the groups. h The percentage of delta power change upon SD during ZT6–12 in Pvalb−2A-Cre:E11-DIO-CN19scr (n = 16) and Pvalb−2A-Cre:E11-DIO-CN19o mice (n = 13). The groups were compared using two-sided Welch’s t-test. Line plots show mean ± SEM. In box plot, boxes show the median, 25th and 75th percentiles, and whiskers show minima to maxima excluding outliers. Source data are provided as a Source Data file.