Fig. 2: Mixotrophic (WT+Fe+Glc) cells are larger, maintain thylakoid membranes and accumulate starch. | Nature Communications

Fig. 2: Mixotrophic (WT+Fe+Glc) cells are larger, maintain thylakoid membranes and accumulate starch.

From: Iron rescues glucose-mediated photosynthesis repression during lipid accumulation in the green alga Chromochloris zofingiensis

Fig. 2: Mixotrophic (WT+Fe+Glc) cells are larger, maintain thylakoid membranes and accumulate starch.

a Light microscopy of cells with Glc and Fe at 84 h. b Transmission electron microscopy (TEM) of whole cells at 96 h showing plastid (p) and starch granules (s). Note that cells were in the dark (during shipping) for ~24 h prior to sampling for TEM so starch differences may have been greater at 84 h. c Detailed TEM of plastid and thylakoids (t), which appear as stacked membranes in −Fe−Glc, +Fe+Glc and +Fe+Glc. Thylakoids in −Fe+Glc are small, fragmented, and difficult to see in −Fe+Glc cultures (see ref. 12. for a 3D reconstruction of thylakoid membranes). d UV-Vis TLC plates of lipid extracted from equal volumetric biomass of Fe and Glc cultures at 84 h and an olive oil TAG standard. Two biological replicates are loaded per treatment and an experimental replicate can be found in Fig. 7a. A complete set of images can be found in Supplementary Data 2 or on the online data repository https://osf.io/r8dbe/. Source data are provided in the Source Data File.

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