Fig. 7: Concerted upregulation of de novo fatty acid synthesis in both WT+Glc proteomes.
a UV-TLC showing high TAG accumulation in WT−Fe+Glc and WT+Fe+Glc at 84 h (equal volumetric biomass loaded with olive oil TAG standard). b Log2 abundance of the major lipid droplet protein 1 (MLDP1) with Glc and Fe at 84 h c Volcano plot results of statistical pipeline to isolate proteins that were significantly highest or lowest in both TAG-accumulating conditions at 84 h (WT−Fe+Glc, WT+Fe+Glc). Proteins that pass statistical threshold are in color with highest (orange) and lowest in TAG (turquoise). The labeled proteins include the manually annotated lipid biosynthesis pathway (blue), glycolysis (brown), and proteins putatively involved in fatty acid desaturation (black, FDX2, CBR1, FAD2, FAD7A). All other axes and plot features are the same as Fig. 4e. d Heatmap of proteins associated with TAG accumulation that were highest-during-TAG (112 proteins) or lowest-during-TAG (39 proteins) across all proteomes and pass significance threshold. e Schematic of induced enzymes that likely play a role in the TAG accumulation mechanism, showing a concerted upregulation of de novo fatty acid synthesis (plastid enzymes), with a complete pathway induced. A subset of proteins involved in TAG condensation were also upregulated. PAP4 was not detected in this study, but its induction has been noted at the transcriptional level in previous studies10,12. Proteins pass statistical significance from volcano are labeled orange. Most orange-yellow proteins were additionally highest in WT+Glc conditions but did not exceed the fold change stringency cut-off. MCT1 and KAS1 (with *) were induced by +Glc in WT but were also induced in one or both hxk1+Glc strains. Source data are provided in the Source Data File.
