Fig. 7: RNA-seq analysis of roots of WT and rem1a inoculated with HH103 or nopL mutant at 24 dpi.
From: The type III effector NopL interacts with GmREM1a and GmNFR5 to promote symbiosis in soybean
a Heat maps of symbiosis-associated genes in WT or rem1a mutants inoculated with MgSO4 (Mock), HH103 or nopL mutants. The scale bar at the bottom of the panel indicates the log2FPKM (Fragments Per Kilobase Million). b Venn diagrams showing unique and common genes amongst differentially expressed genes (DEGs) for different pairwise comparisons. The data of Gmrem1a (HH103) vs. DN50 (HH103) set is shown in blue, and the data of DN50 (nopL) vs. DN50 (HH103) set is shown in yellow. c Scatterplot showing the expression correlation of DEGs in DN50 inoculated with nopL mutants and in Gmrem1a plants inoculated with HH103 compared with DN50 inoculated with HH103. d Scatterplot showing the expression correlation of DEGs in Gmrem1a plants inoculated with HH103 or nopL mutants compared with DN50 inoculated with HH103. The black line is the linear regression. R, Pearson correlation coefficient. FC, Fold change in c, d. e–g, Relative expression level of GmRINRK, GmNINa and GmNFR5 in roots of WT or rem1a mutants inoculated with HH103, nodA mutants, nopL mutants or nodA and nopL double mutant in 24 dpi. Values are means ± SD (n = 3 biological repeats) and P < 0.05 by ANOVA with Tukey’s multiple comparison tests. Source data are provided as a Source Data file.
