Fig. 1: Lysosomes in senescent cells contain increased glycolytic enzymes than in young cells.
From: PKM2 aggregation drives metabolism reprograming during aging process
a schematic diagram shows the process of isolating lysosomes from young and etoposide-induced senescent cells and the subsequent identification and verification. b GSEA plot of core components in glycolysis pathway according to the fold change scores in (a). c The Glycolysis pathway in which the increased glycolytic enzymes in lysosomes of senescent cells were marked in different levels of red. d Lysosome immunoprecipitation and subsequent immunoblotting analysis in HEK 293T (TMEM192-3HA) cells treated with 2 μM etoposide (ETO) for indicated days. e, f Representative images (e) and quantification (f) of PKM2 and lysosomes (indicated by HA staining) in HEK 293T (TMEM192-3HA) cells treated with 2 μM etoposide (ETO) for indicated days. Scale bar, 5 μm. n = 4 (four randomly captured images), one-way ANOVA was used, P = 0.0107 (DMSO vs. ETO Day 1). *P < 0.05, ****P < 0.0001. Error bars represent SEM. Source data are provided as a Source Data file.
