Fig. 3: PKM2 aggregates comprise many glycolytic enzymes.
From: PKM2 aggregation drives metabolism reprograming during aging process
a Confocal microscopy in HeLa cells stably expressed with sfcherry or sfcherry-PKM2. Scale bar, 10 μm. b HeLa cells stably expressed with vector, sfcherry, sfcherry-PKM2 were collected and lysed followed by FACS to isolate PKM2 aggregates. c The proteins with high abundance in the MS results of PKM2 aggregates isolated by FACS. d Schematic view of the PKM2 protein-protein interaction network collected from STRING. Proteins were colored according to the phase separation propensity predicted by PhaSePred. The line between two proteins indicated their interaction gathered from high or low throughput. e Immunoblotting of PKM2 and proteins with high abundance in the MS results of PKM2 aggregates. f, g Immunofluorescent imaging of PKM2 and GAPDH (f) or ENO1 (g) in senescent HEK 293T cells induced by 2 μM etoposide (ETO). Scale bar, 10 μm. h Immunofluorescent imaging of PKM2 and GAPDH in scramble or PKM2 knockdown HEK 293T cells treated with 2 μM etoposide (ETO). Scale bar, 10 μm. All the above experiments were repeated thrice on separate days with similar results. Source data are provided as a Source Data file.
