Fig. 3: Results of a proof-of-principle experiment performed with 26 fluorescent beads including a neuropil background source.
From: Demixing fluorescence time traces transmitted by multimode fibers
The bead #20 is modeling the neuropil (highlighted in orange). From (a) to (d) we have: (a) the photometric (ensemble) time trace, which is the sum of 26 time traces; (b) the sCMOS detected image of the spatially overlapped fingerprint patterns from 26 fluorescent beads probed by the short MMF (see “Methods”); (c) the short MMF located at a distance of 60 ± 10 µm from the sample; (d) the ground truth image of the sample (backpropagated fluorescence image detected from a CMOS Basler camera, see details of the setup in Fig. S1). e The ground truth (GT) fingerprint patterns obtained from each bead when they were individually excited. f The fingerprint patterns obtained via NMF are to be compared with the GT patterns in (e). g Top: the individual temporal activity traces obtained with NMF (blue) and their corresponding GT traces (gray). Bottom: the photometric ensemble signal from the recorded video (black line), which is the sum of all individual traces. The fluorescence intensity in all traces in the figure are normalized to 1. h The GT-NMF time trace correlations. The average diagonal value of the first 22 beads was <δg,n > = δavg = 86.0% with \({\sigma }_{\delta }\) = 5.4%. To better evaluate the off-diagonal elements (time trace cross-talk), we subtract them from their corresponding GT-GT coefficients. Then, we averaged the absolute values of these differences and we obtained the mean cross-talk of \({\zeta }_{{avg}}=\) 4.4% with a standard deviation of\(\,{\sigma }_{\zeta }=\) 3.7% for the first 22 beads (see Supplementary Note 2). i The GT-GT temporal trace correlation table showing that the ground truth traces were not orthogonal.
