Fig. 6: Vegfc overexpression fails to rescue the devoid of BLECs caused by egfl7 mutation. | Nature Communications

Fig. 6: Vegfc overexpression fails to rescue the devoid of BLECs caused by egfl7 mutation.

From: Epidermal growth factor-like domain 7 drives brain lymphatic endothelial cell development through integrin αvβ3

Fig. 6

a Schematic diagram showing the experimental design for detecting the development of LECs after the ectopic, mosaic expression of Vegfc and Venus. Illustrations of the method, transgenic lines, plasmids, and time points of heat shock, and imaging. b, d Compared to the heatshock of Venus, overexpression of Vegfc, causing a hyperbranching in adjacent PLs (arrows) and hyperbranched ISVs in the horizontal myoseptum (arrowheads) of WT (b) and egfl7 mutant (d). f, g The statistics shows the number of PLs per 6 somites (f) and the number of hyperbranched ISVs (g) after ectopic expression of Vegfc and Venus in the trunk (n = 10 embryos; two-tailed unpaired t-test). c, e Compared to the heatshock of Venus, overexpression of Vegfc in the brain does not rescue the devoid of BLECs in the egfl7 mutant. h Quantification of the number of BLECs in the brain after ectopic expression of Vegfc and Venus in the brain (n = 10 embryos; two-tailed unpaired t-test). Data are represented as mean ± SD. Scale bar, 50 μm.

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