Fig. 2: FISH localization of M-loci and sex chromosome-associated repeat regions.
From: Divergent evolution of male-determining loci on proto-Y chromosomes of the housefly
a, b Using an Mdmd-specific probe, MY and MIII were localized to pericentromeric regions of the Y chromosome and chromosome III respectively. c MV was not detected by the Mdmd probe due to insufficient gene copy numbers. d–i Using a probe containing a mixture of amplified MIII sequences including Mdmd and non-Mdmd intervening sequences, the M-locus and the M and sex chromosome-located (MAS) regions of the XY chromosome pair were localized. The signals of the mixed probe on the Y chromosome cover most parts of the short arm and merge with the MY signal. The signal of the mixed probe on the X chromosome mark at the ends of both arms. Positive signals are shown in red and indicated by open triangles, chromosomes are indicated by arrows. Metaphase chromosomes are shown in blue. Signals were only considered as a successful hybridization if they were observed with consistent chromosomal locations on at least 20 metaphase nuclei on each slide. For each strain, 2–3 individuals were tested to ensure reproducibility. Scale bar: 10 μm.
