Fig. 5: Host glutathione, polyamine, and heme pathways are essential for Theileria. | Nature Communications

Fig. 5: Host glutathione, polyamine, and heme pathways are essential for Theileria.

From: Host cell CRISPR genomics and modelling reveal shared metabolic vulnerabilities in the intracellular development of Plasmodium falciparum and related hemoparasites

Fig. 5

a–c Scoring of GCLC, GSS, AMD1, SMS, SRM, ALAD, HMBS, UROS, CPOX, and FECH in TaC12 vs BoMac screens. TIDE analysis of GSS, SRM, and HMBS CRISPR/Cas9 knockout in BoMac and TaC12 cells (red, WT; green, frameshift mutation; light blue, in-frame mutation; black, not determined). The gene knocked out is indicated with delta (Δ) in the corresponding schematic biosynthetic pathway. Resazurin viability assay showing the percentage of survival of BoMac and TaC12 cells after 72 h treatment with three different enzymatic inhibitors: buthionine sulfoximine (BSO) (inhibitor of γ-GCS), sodium nitroprusside (SNP) (inhibitor of ODC), and salicylic acid (SA) (inhibitor of FECH). TIDE and viability experiments were performed in triplicate with similar results (Fig. S8a–e). A representative replicate is shown for each.

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