Fig. 7: DHX9 SUMOylation enhances its association with PARP1 and DDX21 via SUMO-SIM interaction.

a Schematic diagrams show predicted SIMs in PARP1, and a previously identified SIM in DDX21, with their locations highlighted in red boxes. b HeLa cells co-transfected with indicated constructs were split into two groups. One for FLAG affinity purification and the other for HA affinity purification. The proteins precipitated were determined by Western blot, and the quantification data are presented as mean ± SD from three separate experiments analyzed by two-sided t test. c, d HeLa cells co-transfected with indicated constructs were used for IP. One group of samples were subjected to FLAG affinity purification (c) and the other group of samples were subjected to HA affinity purification (d). The association of SFB-DHX9 with HA-DDX21 variants was determined by Western blot, and the quantification data are presented as mean ± SD from three separate experiments analyzed by a two-sided t test. e The SUMO pathway modulates protein associations between SFB-DHX9 and multiple RNA-processing factors. HeLa cells transfected with siControl or siUBC9 were transfected with indicated constructs, followed by FLAG affinity purification. Associations between DHX9 and RNA-processing factors were determined by Western blot (n = 3). Source data are provided as a Source Data file.