Fig. 5: DmxA is essential for the symmetric incorporation and allocation of the core T4PM proteins at the nascent and new cell poles. | Nature Communications

Fig. 5: DmxA is essential for the symmetric incorporation and allocation of the core T4PM proteins at the nascent and new cell poles.

From: A deterministic, c-di-GMP-dependent program ensures the generation of phenotypically similar, symmetric daughter cells during cytokinesis

Fig. 5: DmxA is essential for the symmetric incorporation and allocation of the core T4PM proteins at the nascent and new cell poles.The alternative text for this image may have been generated using AI.

a PilQ-sfGFP localization during the cell cycle. Left panels, epifluorescence and phase-contrast images from time-lapse microscopy of representative WT and ΔdmxA cells. Images were recorded every 30 min; arrowheads indicate completion of cytokinesis. Right panels, quantification of PilQ-sfGFP localization pattern based on time-lapse microscopy. PilQ-sfGFP polar fluorescence was analysed before and after division in the predivisional cell (gray) and the daughters (yellow and pink) and the asymmetry index (ω) calculated as indicated; the pole with the highest fluorescence is defined as Pole 1. Daughter cells are sorted based on their ω with the most symmetric in yellow and the other in pink. Line and error bars, mean ± SD from three biological replicates, number of cell division events per replicate in brackets. Schematics show dominant localization patterns of PilQ-sfGFP in WT and ΔdmxA cells during the cell cycle. b Localization of PilQ-sfGFP, mCherry-PilM, mCherry-PilT and PilB-mCherry in WT and ΔdmxA cells. Left panels, representative snapshot images. Scale bar, 5 μm. Middle panels, histograms of the distribution of the fraction of total cellular fluorescence in polar clusters at pole 1 and pole 2 in WT (gray) and ΔdmxA (blue) cells. The pole with the highest fluorescence is defined as Pole 1. Numbers in the upper right corners indicate the median±MAD fluorescence signal at pole 1 (P1) and pole 2 (P2). The fraction of cells with no polar signal(s) is indicated in the leftmost column labeled d (for diffused) in green; cells in which no polar cluster was detected at pole 1 also do not have a signal at pole 2. Right panels, histograms of the distribution of ω. Localization patterns are binned as unipolar, asymmetric, and symmetric from the ω values as indicated; cells in which no polar signal was detected were not considered in the analysis. Error bars indicate median ± MAD. Differently colored circles indicate the median of each of the three biological replicates. The total number of analysed cells is indicated below. Samples were analysed using the two-sided Mann–Whitney test. Source data are provided as a Source Data file.

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