Fig. 1: Aberrant MLKL elevation-mediated necroptosis is associated with liver metastasis of early T stage PDAC. | Nature Communications

Fig. 1: Aberrant MLKL elevation-mediated necroptosis is associated with liver metastasis of early T stage PDAC.

From: Necroptosis enhances ‘don’t eat me’ signal and induces macrophage extracellular traps to promote pancreatic cancer liver metastasis

Fig. 1: Aberrant MLKL elevation-mediated necroptosis is associated with liver metastasis of early T stage PDAC.

a The overall experimental scheme used in this study. b MRI of T1M1 (n = 6) and T1M0 (n = 6) PDAC patients. Yellow arrow: liver metastasis; yellow circle: primary pancreatic tumour. c Matrix diagram of RNA-seq data from T1M0-PDAC (n = 6) and T1M1-PDAC (n = 6) samples. d Venn diagram illustrating the overlap between differentially expressed genes (DEGs) between the tumour and normal groups and the T1M1 and T1M0 PDAC groups. e KEGG pathway enrichment analysis of DEGs between the T1M0 and T1M1 PDAC groups. f Volcano plot of the final 216 DEGs. g KEGG pathway enrichment analysis of the final 216 DEGs. h Heatmap of clinicopathological features, genetic profiles, and survival information among groups with different MLKL expression levels (n = 170) in the cohort from our centre. The two-sided Pearson chi-square test and were used to assess the differences between the two groups; overall survival (OS) probability was compared using log-rank test, two-sided. i Representative images of immuno-stained MLKL and p-MLKL(T357/S358/S360) in the PDCA cohort (n = 20) from our centre; Scale bar: 50 μm. j WB and quantification of necroptosis markers in tumour samples from patients with T1M0-PDAC (n = 6) and T1M1-PDAC (n = 6). k Representative images following H&E staining and IHC staining (MLKL) of T1M0 and T1M1 PDAC primary tumour organoids. Organoids were constructed from primary tumours that performed RNA-seq of T1M1 and T1M1 PDAC patients who underwent MRI. n = 6 biologically independent samples for each group, Scale bar: 50 μm. Unless specified otherwise, the data are presented as means ± SEM (error bar) and compared using the two-sided Student’s t test; *P < 0.05; **P < 0.01; and ***P < 0.001; ns, no significance. a Created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license (https://creativecommons.org/licenses/by-nc-nd/4.0/deed.en). Source data are provided as a Source Data file.

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