Fig. 2: Reaction-diffusion model with a single condensate.
From: Multi-condensate state as a functional strategy to optimize the cell signaling output
A Illustration of the simulation geometry. The “Virtual Cell” software is used to define the geometry and set up the simulation conditions. The hemispherical condensate is situated on the XY plane. Arp2/3 only diffuses around the condensate surface. Actins (both G and F) can diffuse in the entire region. B Concentration profiles (shown as color bars) of the model components at the beginning of simulations (Time = 0.2 s, upper panel) and the steady state (Time = 20 s, lower panel). These snapshots represent the plane at Z = 0. Diffusion constants, DGA = 10 µm2/s and DFA = 1 µm2/s. C Timecourse of F-actin (FA) concentration at two FA diffusion constants (DFA). To compute the “Surface concentration”, a hemispherical region of interest (larger than the condensate) is considered (details in method) and the amount of FA in the shell volume measures the surface concentration. The “Bulk concentration” incorporates all the volume elements, including the condensate surface. D Measure of local production of F-actin as a function of differential diffusion of actin in monomeric (DGA) and polymeric (DFA) form. We keep DGA (10 µm2/s) fixed and vary DFA. E Trend of local FA production as a function of the condensate’s HemiSphericity.
