Fig. 3: Reaction-diffusion model with multiple condensates.
From: Multi-condensate state as a functional strategy to optimize the cell signaling output
A Illustration of the simulation setup. This snapshot from the “Virtual Cell” displays four hemispherical condensates situated at the XY plane. Similar to Fig. 2A, Arp2/3 only diffuses around the condensate surface. Actins (both G and F) can diffuse in the entire region. B Concentration profiles (shown as color bars) of the model components at the steady state (Time = 20 s, last timepoint). These snapshots represent the plane at Z = 0. DGA = 10 µm2/s and DFA = 1 µm2/s. C Timecourse of F-actin (FA) concentration at two FA diffusion constants (DFA). “Local” refers to the F-actin (FA) produced at each condensate surface, while “Global” is the cumulative FA produced at four-condensate surfaces. To compute the surface concentration, as in Fig. 2, a hemispherical region of interest (larger than the condensate) is considered (detailed in Method). The black dotted line (“single cluster limit”) indicates the FA concentration (surface FA from Fig. 2C) when an equivalent amount of Arp2/3 is concentrated on the surface of a single large condensate. D Quantification of relative gain (in FA production) as a function of differential diffusion of actin in monomeric (DGA) and polymeric (DFA) form. FA4Global indicates the global FA concentration (blue lines in C) in the four-cluster state, while FA1 is the single-cluster limit. E Quantification of FA production gain in a four-condensate state as a function of individual condensate’s HemiSphericity.
