Fig. 6: Spatial tuning in pyramidal cells dendrites following place field induction.
From: Functional architecture of intracellular oscillations in hippocampal dendrites
a Experimental setup. ASAP3, mRuby3 and bReaChes plasmids were single-cell electroporated in CA1 pyramidal cells. Place fields were optogenetically induced with 1s-long LED photostimulation of bReaChes at randomly chosen, fixed location on the treadmill belt for five consecutive laps. b Example tuning maps of dendrites from a non-induced (non-place) and an induced (place) cell, sorted by tuning curve maximum. c Mean normalized DE tuning vectors from an example non-induced (non-place) and an induced ('place') cell. Each arrow represents a single dendrite. d Tuning diversity across the dendritic arbor (σarbor) in induced (N = 15) and non-induced (N = 7) cells. Top: The dendritic arbor of induced cells exhibit significantly less tuning diversity σarbor, defined as the circular standard deviation of tuning peaks in the arbor, compared to the dendrites of induced cells (two-sided Mann–Whitney U test, p = 8 × 10−5). Bottom: Relationship between arbor tuning diversity and strength of somatic tuning. The arbors of induced cells exhibit low diversity at all somatic tuning strengths, while a significant inverse relationship exists between tuning diversity and soma tuning strength in non-induced cells (r2 = 0.74, p = 0.006). Box: Q1, Q2, Q3; whiskers: range excluding outliers. e Relationship between tuning parameters (tuning peak, tuning strength) at the level of individual soma-dendrite pairs in non-induced (N = 7) and induced (N = 15) cells. Top row: Dendrite peak locations are tightly coupled to their corresponding soma peak location (r2 = 0.95 for the line y = x, black dashed line; N = 123 dendrites from 15 non-induced cells) in induced cells but much less coherent (r2 = −0.63, N = 217 dendrites from 7 cells) in non-induced cells. Bottom row: More strongly tuned somas tend to have dendrites with more spatially coherent firing in both non-induced (r2 = −0.22) and induced (r2 = 0.44) cells, with a higher magnitude correlation for induced compared to non-induced cells. Regression fit line is shown with bootstrapped 95% CI.
