Fig. 5: FAM92A1 depletion perturbs neuronal function.
a Representative image showing GCaMP6m expression in mouse HPC. b, c Heatmaps and line graph showing the calcium signals in hippocampal neurons of freely behaving mice. Data represent mean ± SEM; recordings (n = 9 for FAM92A1+/+ group, n = 10 per group for FAM92A1+/− and FAM92A1−/− groups) from two to five mice per group. d, e (Left) representative traces of sEPSC (d) and mEPSC (e) in granule neurons of hippocampal DG region. (Right) Bar graph showing the changes in the frequency and amplitude during the recording of sEPSC (d) and mEPSC (e). Data present mean ± SEM; n = 9 neurons for FAM92A1+/+ group and n = 11 neurons for FAM92A1−/− group (d), n = 9 neurons per group (e) from three mice per group; unpaired two-tailed Student’s t-test. f Representative bright-field images of primary hippocampal neurons grown on MEA plate at DIV10 from five MEA wells per group. Scale bar, 100 μm. g Activity heatmaps showing spike rate (spikes per second). h Traces of voltage showing neuronal spontaneous activity. i, j Box plots showing the number of spikes per minute (i) and mean firing rate (Hz) (j). k Mean plot showing the number of active electrodes, bursting electrodes, and electrodes participating in bursts. l Raster plots showing neuronal network activity in a 30 sec time frame. The gray bars represent the detected network bursts. m (Top) Cross-correlogram showing synchronous activity. (Bottom) Box plot showing the synchrony index. n–p The number of network bursts per minute (n) and spikes per network bursts (o), and the duration (sec) of bursts (left) and network bursts (right) (p). Data presented as minimum to maximum values (whiskers) and the 25th to 75th percentiles (box), with the mean value within the box (i, j, m, and o) and data represent mean ± SEM (k, n, and p); n = 5 MEA wells per group; multiple unpaired two-sided t-tests. *p < 0.05, **p < 0.01, and ***p < 0.001. Source data and exact p-values are provided as a Source Data file.
