Fig. 1: Design and test of split intein constructs to assemble FL-dystrophin.
From: Systemic delivery of full-length dystrophin in Duchenne muscular dystrophy mice
a Diagram showing the domain structure of FL-dystrophin, the two split sites, the relative initiation sites of Dp260 and Dp140 isoforms, and the three split fragments fused with inteins (Dys-N1, M1, and C1). The antigen epitopes for three different dystrophin antibodies (anti-N, anti-M, and anti-C) were also labeled. b, Western blotting analysis of dystrophin expression in HEK293 cells transfected with or without the Dys-N1, M1, and C1 constructs at different molar ratios. WT mouse skeletal muscle lysate was loaded as a positive control and the GAPDH was used as a loading control. This experiment was repeated independently three times with similar results. Source data are provided as a Source Data file.
