Fig. 1: Non-spherocytic hemolytic anemia with increased reticulocytes and splenomegaly in Klf1H350R/- mice. | Nature Communications

Fig. 1: Non-spherocytic hemolytic anemia with increased reticulocytes and splenomegaly in Klf1H350R/- mice.

From: Mutations in linker-2 of KLF1 impair expression of membrane transporters and cytoskeletal proteins causing hemolysis

Fig. 1

A Peripheral blood stained by May-Grünwald Giemsa (MGG) reveal hypochromic microcytic red blood cells (RBC) with anisopoikilocytosis (red arrows) and nucleated RBC (black arrow) in compound heterozygous mice (Klf1H350R/-). B Compound heterozygote mice have increased reticulocytes, decreased mean corpuscular volume (MCV), decreased hemoglobin content, and decreased hematocrit levels compared to wild type. C Compound heterozygous animals display a significant increase in the proportion of CD71-positive cells in blood and a significant decrease of TER119-positive cells in the bone marrow compared to normal mice. There is an expansion of red pulp (rp), but not white pulp (wp) in the spleens of compound heterozygous mice (D) together with an increase in relative spleen weight (E). F Spleens of compound heterozygous animals show a relative expansion of erythroid cells. In panels (B) and (E), each dot represents an independent animal (n ≥ 3) with bars representing mean ± SEM (***P < 0.0005, **P < 0.05). One-way ANOVA with Tukey post-hoc test was used for all statistical analysis. Images were taken on an Olympus BX50 with a DP26 camera using the cellSens Standard software. Scalebars represent either 20 μM or 100 μM as indicated.

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