Fig. 1: Nitrogen downshifts lead to phenotypic heterogeneity.
From: Phenotypic heterogeneity follows a growth-viability tradeoff in response to amino acid identity
a Conditions tested throughout this study. Cells were grown in rich (YPD) media until they reached exponential phase, washed twice with PBS and resuspended in one of the media. Different modalities (unimodal or bimodal) were observed across conditions. Flow cytometer and microscope schematics were made with BioRender.com released under a CC-BY-NC-ND 4.0 International license. b Pipeline used throughout this study to monitor single-cell differentiation. c scRNAseq datasets were obtained from ref. 23 and describe cells shifted to 0.8 mM proline (NLIM-PRO) or glutamine (NLIM-GLN) as well as the control (YPD). Plots represent dimensionality-reduced data using UMAP, where each point represents a single cell. Cells in the top UMAP plot are coloured by growth scores, calculated from a regression model39 trained on bulk RNAseq data. Histogram above represents the density of growth scores (GS) for each condition. d Flow cytometry data for cells exposed to a nitrogen downshift. GFP fluorescence (BL1-H channel) and cell size (FSC-H forward scatter channel) were used to measure single-cell heterogeneity. Arbitrary units are shown (abbreviated a.u.).
