Fig. 6: Panx1 inhibition reduces cue-induced morphine seeking behaviours. | Nature Communications

Fig. 6: Panx1 inhibition reduces cue-induced morphine seeking behaviours.

From: Pannexin-1 channel inhibition alleviates opioid withdrawal in rodents by modulating locus coeruleus to spinal cord circuitry

Fig. 6: Panx1 inhibition reduces cue-induced morphine seeking behaviours.

A Schematic representation of morphine self-administration paradigm in Long Evans rats (illustration adapted from Markovic et al.45). B Progress of extinction in morphine seeking behaviours treated with probenecid (PRB, 100 mg/kg, i.p.) vs. saline (SAL) (SAL + SAL: N = 33, SAL + PRB and PRB + PRB: N = 14, PRB + SAL: N = 11 rats). C Progress of extinction in morphine seeking behaviours treated with EG-2184 (EG, 0.5 mg/kg, i.p.) vs. vehicle (VEH). (EG + EG: N = 8, VEH + VEH, VEH + EG, EG + VEH: N = 9 rats). D Effect of PRB (100 mg/kg, i.p.) administered during extinction (EXT, PRB + SAL), reinstatement (RST, SAL + PRB), or during both periods (PRB + PRB) on morphine cue-induced reinstatement (two-way ANOVA, time: p < 0.0001, PRB: p = 0.0002, SAL + SAL: N = 33, SAL + PRB and PRB + PRB: N = 14, PRB + SAL: N = 11 rats). E Effect of EG-2184 (0.5 mg/kg, i.p.) administered during extinction (EXT, EG + VEH), reinstatement (RST, VEH + EG), or during both periods (EG + EG) on morphine cue-induced reinstatement (two-way ANOVA, time: p = 0.0005, EG-2184: p = 0.2776, interaction: p = 0.0367, EG + EG: N = 8, VEH + VEH, VEH + EG, EG + VEH: N = 9 rats). All graphed data are presented as mean values ± SEM. ***p < 0.001, ****p < 0.0001. Source data are provided as a Source Data file.

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